Journal: Gastroenterology Research and Practice

Article Title: Evaluating IL-21 as a Potential Therapeutic Target in Crohn's Disease

doi: 10.1155/2018/5962624

Figure Lengend Snippet: Prophylactic treatment of AdTr mice with anti-IL-21 mAb ameliorates colitis. Open circles indicate individual anti-IL-21 mAb-treated mice, black squares indicate individual mIgG1-treated mice, and grey triangles indicate individual control SCID mice. Delta body weight change over time (a). White blood cell count on day 28 (b). Frequency of CD4 + TCRb + T cell in blood on day 28 (c). Colon weight : length ratio (d). Endoscopic score encompassing (thickening of the colon, changes of vascular pattern, visible fibrin, granularity of mucosal surface, and stool consistency) on day 28 (e). Pictures showing representing images of the colon in anti-IL-21 mAb-treated mice (left), mIgG1-treated mice (middle), and SCID control mice (right) (f).

Article Snippet: C.B-17 severe combined immunodeficiency (SCID) mice, BALB/c, NMRI, C57BL/6NTac, B6.129.S6Rag2tm1FwaN12 mice, IL-21R −/− C57BL/6, and IL-21R +/+ C57BL/6 littermates were purchased from Taconic M&B (Ry, Denmark).

Techniques: Control, Cell Counting

Journal: Gastroenterology Research and Practice

Article Title: Evaluating IL-21 as a Potential Therapeutic Target in Crohn's Disease

doi: 10.1155/2018/5962624

Figure Lengend Snippet: Interventive treatment of AdTr mice with anti-IL-21 mAb. Open circles indicate individual anti-IL-21 mAb-treated mice, black squares indicate individual mIgG1-treated mice, and grey triangles indicate individual control SCID mice. Delta body weight change over time (days) (a). White blood cell count on days 21 and 34 (b). Frequency of CD4 + TCRb + T cells in blood on day 34 (c). Colon weight : length ratio (d). Endoscopic score encompassing (thickening of the colon, changes of vascular pattern, visible fibrin, granularity of mucosal surface, and stool consistency) on days 21, 34, and 47 (e).

Article Snippet: C.B-17 severe combined immunodeficiency (SCID) mice, BALB/c, NMRI, C57BL/6NTac, B6.129.S6Rag2tm1FwaN12 mice, IL-21R −/− C57BL/6, and IL-21R +/+ C57BL/6 littermates were purchased from Taconic M&B (Ry, Denmark).

Techniques: Control, Cell Counting

Journal: Gastroenterology Research and Practice

Article Title: Evaluating IL-21 as a Potential Therapeutic Target in Crohn's Disease

doi: 10.1155/2018/5962624

Figure Lengend Snippet: Cytokine and chemokine profile in inflamed colon from mice treated with Anti-IL-21 mAb or mIgG1. Colon biopsies isolated from individual mice with AdTr colitis. Open circles anti-IL-21 25 mg/kg, black squares mIgG1, and grey triangles SCID controls. KC (a), CCL3 (b), CCL5 (c), IL-17 (d), TNF α (e), IFN- γ (f), CCL2 (g), and IP-10 (h) were found to be significantly downregulated in anti-IL-21 mAb-treated mice.

Article Snippet: C.B-17 severe combined immunodeficiency (SCID) mice, BALB/c, NMRI, C57BL/6NTac, B6.129.S6Rag2tm1FwaN12 mice, IL-21R −/− C57BL/6, and IL-21R +/+ C57BL/6 littermates were purchased from Taconic M&B (Ry, Denmark).

Techniques: Isolation